Biosynthesis of dicarboxylic acids by carbon dioxide fixation. IV. Isolation and properties of an adaptive "malic" enzyme from Lactobacillus arabinosus.
نویسندگان
چکیده
Reaction 1 requires DPN and manganous ions. It is specific for Imalic acid; d-malic acid is inactive. TPN cannot replace DPN. Reaction 2 requires the presence of manganous ions. The evidence presented below supports the view that Reaction 1 is a DPN-linked dismutation between Z-malate and pyruvate, resulting from the interaction of lactic dehydrogenase with a DPN-specific “malic” enzyme as shown by Reactions 3 and 4.
منابع مشابه
Biosynthesis of dicarboxylic acids by carbon dioxide fixation; isolation and properties of an enzyme from pigeon liver catalyzing the reversible oxidative decarboxylation of 1-malic acid.
followed spectrophotometrically. The spectrophotometric method could not be applied to the determination of the equilibrium constant of Reaction 1 owing to interference by lactic dehydrogenase which contaminated the best preparations of the pigeon liver enzyme available (1). However, through coupling with the isocitric dehydrogenase system (Reaction 2), Reaction 3 was obtained (3). The equilibr...
متن کاملBiosynthesis of dicarboxylic acids by carbon dioxide fixation. II. Further study of the properties of the "malic" enzyme of pigeon liver.
This enzyme is provisionally referred to as “malic” enzyme. The present paper deals with further observations on the kinetics and other properties of the purified enzyme.’ The enzyme is totally inactive in the absence of Mn++. Mg++ can replace Mn++ but is less effective. Further evidence has been obtained indicating that free oxalacetate is not an intermediate in Reaction 1 and that both Reacti...
متن کاملThe influence of biotin nutrition on carbon dioxide fixation and malic enzyme formation by Lactobacillus arabinosus.
Biotin has long been impliratcld in a number of carbon dioxide fixation reactions (see Lardy and Peanasky (1) for rcvicw). Two main proposals have been made to explain its mode of action: 1. The vitamin acts as a coenzyme in carbon dioxide fixation reactions. This view was based mainly on observations on the incorporation of radioactivity from C?“oz into compounds in equilibrium with certain su...
متن کاملBiosynthesis of dicarboxylic acids by carbon dioxide fixation. III. Enzymatic synthesis of iota-malic acid by reductive carboxylation of pyruvic acid.
A complete chemical balance which demonstrates the occurrence of Reaction 3 has now been obtained. There is no reaction in the absence of either TPN, .Zwischenferment, or “malic” enzyme. The rate of reaction is proportional to the concentration of “malic” enzyme and to the CO* tension. It is also possible to couple the isocitric dehydrogenase-oxalosuccinic carboxylase system (2) with “malic” en...
متن کاملThe Preparation of C4-labeled Biotin and a Study of Its Stability during Carbon Dioxide Fixation by Donald B. Melville,
Several lines of evidence indicate that biotin is involved in the biological fixation of carbon dioxide. Lardy, Potter, and Elvehjem (1) have demonstrated that the biotin requirement of Lactobacillus arabinosus is partially replaceable by oxalacetate and that the growth stimulation of this organism by carbon dioxide is evident only when biotin is present. From microbiological studies with antim...
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ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 187 2 شماره
صفحات -
تاریخ انتشار 1950